Reagents for gene transfection
نویسندگان
چکیده
منابع مشابه
Optimization of Gene Transfection in Murine Myeloma Cell Lines using Different Transfection Reagents
Purification and isolation of cellular target proteins for monoclonal antibody (MAb) production is a difficult and time-consuming process. Immunization of mice with murine cell lines stably transfected with genes coding for xenogenic target molecules is an alternative method for mouse immunization and MAb production. Here we present data on transfection efficiency of some commercial reagents us...
متن کاملoptimization of gene transfection in murine myeloma cell lines using different transfection reagents
purification and isolation of cellular target proteins for monoclonal antibody (mab) production is a difficult and time-consuming process. immunization of mice with murine cell lines stably transfected with genes coding for xenogenic target molecules is an alternative method for mouse immunization and mab production. here we present data on transfection efficiency of some commercial reagents us...
متن کاملThe intracellular trafficking mechanism of Lipofectamine-based transfection reagents and its implication for gene delivery.
Lipofectamine reagents are widely accepted as "gold-standard" for the safe delivery of exogenous DNA or RNA into cells. Despite this, a satisfactory mechanism-based explanation of their superior efficacy has remained mostly elusive thus far. Here we apply a straightforward combination of live cell imaging, single-particle tracking microscopy, and quantitative transfection-efficiency assays on l...
متن کاملStructural characteristics of supramolecular assemblies formed by guanidinium-cholesterol reagents for gene transfection.
We have recently discovered that cationic cholesterol derivatives characterized by guanidinium polar headgroups are very efficient for gene transfection in vitro and in vivo. In spite of being based on some rationale at the molecular level, the development of these new synthetic vectors was nevertheless empirical. Indeed, the factors and processes underlying cationic lipid-mediated gene transfe...
متن کاملComparison of Commercial Transfection Reagents: Cell line optimized transfection kits for in vitro cancer research
The process of in vitro transfection involves introduction of genetic material into cells and it is generally used for mammalian cells and involves non-viral methods [1]. Various therapeutic cargo molecules can be used for intracellular delivery into cancer cell lines and primary cells, including plasmid DNA (pDNA), proteins, small molecules, messenger RNA (mRNA), shall RNA such as short interf...
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ژورنال
عنوان ژورنال: Drug Delivery System
سال: 2017
ISSN: 0913-5006,1881-2732
DOI: 10.2745/dds.32.66